Mass Spectrometry Facility
Model: Orbitrap Fusion Tribrid mass spectrometer, Thermofisher Scientific
Principle
Mass spectrometry (MS) is an analytical method in which sample produces charged ions according to the complexity of the sample and further they are determined according to their mass and charge. A spectrum is produced during sample acquisition which shows ion abundance versus m/z ratio. A mass spectrometer analyze the samples in following components
• Ionization source -Produce charged ions from the sample
• Mass analyzer- resolve these charged ions according to their mass to charge ratio.
• Ion detectors - measures the relative abundance of the ions and transforms the ions into electrical signals.
• Transfer the signals to the computer and data analysis
Applications
• Mass-spectrometry technique helps in qualitative and quantitative analysis of the samples.
• Help in categorizing anonymous compounds in the samples
• Finding the isotopic pattern of the compound
• Identification of post translational modifications
• Screening of metabolites
Specifications
Orbitrap Fusion: Linear Ion Trap and Orbitrap Mass Range (Standard – m/z 50–2,000, High Mass – m/z 200–4,000), Resolution of 15,000-450,000 at m/z 200, Scan speed at 20 Hz, Polarity Switching, Synchronous Precursor Selection
Contact
aef@thsti.res.in
| Equipment Details | Price | ||
|---|---|---|---|
| Government Organisation | Non-Government Organisation | ||
| Mass Spectrometry Facility, Thermofisher Scientific | Rs.5000 | Rs.7000 | |