The proteomics facility at THSTI, operating under the DBT-SAHAJ National Infrastructure Programme, extends access to its cutting-edge LC-HRAM mass spectrometry platforms for biotech and life sciences researchers in academia and industry across India.

Principle

Mass spectrometry (MS) is an analytical method in which sample produces charged ions according to the complexity of the sample and further they are determined according to their mass and charge. A spectrum is produced during sample acquisition which shows ion abundance versus m/z ratio. A mass spectrometer analyse the samples in following components

• Ionization source --Produce charged ions from the sample
• Mass analyzer-- resolve these charged ions according to their mass to charge ratio.
• Ion detectors -- measures the relative abundance of the ions and transforms the ions into electrical signals.
• Transfer the signals to the computer and data analysis

Applications

•  Mass-spectrometry technique helps in qualitative and quantitative analysis of the samples.
•  Help in categorizing anonymous compounds in the samples
•  Finding the isotopic pattern of the compound
•  Identification of post translational modifications

Services

• Protein identification
• Label-free quantitation (DDA and DIA/SWATH)
• Label-based quantitation (SILAC and iTRAQ/TMT)
• Targeted proteomics (SRM/MRM)