Luminex’s xMAP® instrument: Magpix System

Principle

The Magpix system is the combination of three core xMAP® Technologies. xMAP microspheres, a family of fluorescently dyed micro-sized magnetic microspheres, act as both the identifier and the solid surface to build the assay. The second is a flow cytometry-based instrument, the Magpix analyzer, which integrates key xMAP detection components (e.g. light-emitting diodes (LEDs) for excitation, optics, fluidics than detects and analyses the beads using a CCD camera and followed by high-speed digital signal processors). The third component is the xPONENT® software, which is designed for protocol-based data acquisition with robust data regression analysis. The system enables multiplexing of up to 50 analytes in a single microplate well using very small samples. The user can do wide variety of multiplexing assay of protein or nucleic acid assays quickly with more precision and cost effectively.

Applications:

The Magpix system can be used to perform:
Reporter gene assays (including dual-reporter assays)       

ATP assays, kinase activity assays, apoptosis assays                   

Cell proliferation, cytotoxicity, and biomass assays
Nucleic acid probe assays

Luminescent immunoassays                     

Environmental toxicity and mutagenicity assays

Specifications:

Multiplexing: Up to 50 analytes per sample with magnetic beads, Read time: 96 well plate in ≤60 min (up to 4800 tests/hour)

Reporter LED excitation: 532 nm; Classification LED excitation: 621 nm

Reporter detector: CCD Imager, detection bandwidth of 565-585 nm

Classification detector: Avalanche photodiodes with temperature compensation

Contact:

aef@thsti.res.in