eTHSTI : Scientific Equipment

Employee details

Designation Center/Lab

Mobile Email

PI Deatils Project Code

No of Samples Samples ID

Samples Description

*In case there is any modification in above values, pleae contact administration.

Name of the Cell Line/Primary Cells

Supernatant

Others(Specify)

Animal Mouse Rat Other

Indection / Disease Non-Communicable Other

Normal Healthy Patient Sample Type

Glycolysis Kerb's Cycle HMP Shunt Lipidomics Amino acids Nuceotides Any Other Brief description of the experiment

Additional Details

LCMS Grade water Yes No

LCMS grade methanol Yes No

LCMS grade acetonitrile Yes No

C18 column Yes No

HILIC column Yes No

Standards Yes No

This information would help in selecting the column requirement (Important for PI)

Untargeted (Discuss the plan before initiating the experiment)

Method of Sample prepration (in Brief)

Solvent(s) used:

Please do not use following. If necessary, discuss sample prepration with Dr. Yashwant:

Nonvolatile salts (Phosphate, borate, citrate, etc.)
Surface-active agents (Surfactants/detergents) suppress electrospray ionization
Inoroganic acids are corrosive
Trifluoroacetic acid (TFA), Triethylamine (TEA), Tetrahydrofuran (THF), DMSO (Less than 2%)

Results/ Chromatograms shall be provided by the metabolomics facility to the PI through online system.

All the users must Submit Pen Drive (2GB for 50 Samples) and collect the data within 15 days Acquired Data would be deleted at end of each month.