PROFILE PREVIEW

Research Interests/ Research Summary

• Establishment and Support of multidisciplinary translation research platform for Therapeutic Strategies of infectious diseases

Awards and prize

• “Dr D. L. Shrivastava” Young Scientist Award, Society of Biological Chemistry, India (2008)

Publications

• Comparative evaluation of SARS-CoV-2 IgG assays in India. Susmita Chaudhuri, Ramachandran Thiruvengadam, Souvick Chattopadhyay, Farha Mehdi, Pallavi Kshetrapal, Tripti Shrivastava, Bapu Koundinya Desiraju, Gaurav Batra, Gagandeep Kang,* Shinjini Bhatnagar,* and DBT India Consortium for COVID-19 Research, J Clin Virol. 2020 Oct; 131: 104609 [IF:2.777]
• Design and characterization of a germ-line targeting soluble, native-like, trimeric HIV-1 Env lacking key glycans from the V1V2-loop. Shubbir Ahmed, Ph.D; Tripti Shrivastava; Rajesh Kumar; Mohit Kumar; Manidipa Banerjee; Naresh Kumar; Manish Bansal; Supratik Das; Sweety Samal. BBA accepted for publication [IF:3.67]
• Tetramerizing tGCN4 Domain Facilitates Production of Influenza A H1N1 M2e Higher Order Soluble Oligomers that Show Enhanced Immunogenicity In vivo. Sweety Samal, Tripti Shrivastava, Praveen Sonkusre, Zaigham Abbas Rizvi, Rajesh Kumar, Shubbir Ahmed, Preeti Vishwakarma, Naveen Yadav, Manish Bansal, Kanchana Chauhan, Sebanta Pokhrel, Supratik Das, Padmakar Tambare, and Amit Awasthi. JBC accepted for publication. [IF:4.125]
• Identification of an anti-SARS-CoV-2 receptor binding domain directed human monoclonal antibody from a naïve semi-synthetic library. Hilal Ahmed Parray, Adarsh Kumar Chiranjivi, Shailendra Asthana, Naveen Yadav, Tripti Shrivastava, Shailendra Mani, Chandresh Sharma, Preeti Vishwakarma, Supratik Das, Kamal Pindari, Subrata Sinha, Sweety Samal, Shubbir Ahmed, Rajesh Kumar. J Biol Chem, 2020 Jul 29;jbc.AC120.014918.  doi: 10.1074/jbc.AC120.014918. [IF:4.125]
• Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives. Hilal Ahmed Parray, Shivangi Shukla, Sweety Samal, Tripti Shrivastava, Shubbir Ahmed, Chandresh Sharma, Rajesh Kumar. International Immunopharmacology,  Volume 85, August 2020, 106639. [IF: 3.380]
• Antibody-based therapeutic interventions: possible strategy to counter chikungunya viral infection Rajesh Kumar*, Tripti Shrivastava, Sweety Samal and Shubbir Ahmed.. Applied Microbiology and Biotechnology. 2020 Apr;104(8):3209-3228. [IF: 3.670]
• Phage display antibody libraries: A robust approach for generation of recombinant human monoclonal antibodies. Kumar R, Parray HA, Shrivastava T, Sinha S, Luthra K. Int J Biol Macromol. 2019 Aug 15;135:907-918. doi: 10.1016/j.ijbiomac.2019.06.006. Epub 2019 Jun 3. Review.
• HIV Research for Prevention 2018: From Research to Impact Conference Summary and Highlights. Shacklett BL, Blanco J, Hightow-…….. Shrivastava T, Tomaras G, Van Diepen M, Walia M, Warren M, Manrique A, Thyagarajan B, Torri T. AIDS Res Hum Retroviruses. 2019 Jul;35(7):598-607. doi: 10.1089/AID.2019.0074. Epub 2019 May 29.
• Cell surface ectodomain integrity of a subset of functional HIV-1 envelopes is dependent on a conserved hydrophilic domain containing region in their C-terminal tail. Samal S, Das S, Boliar S, Qureshi H, Shrivastava T, Kumar N, Goswami S, Bansal M, Chakrabarti BK. Retrovirology. 2018 Jul 20;15(1):50. doi: 10.1186/s12977-018-0431-4.
• Envelope proteins of two HIV-1 clades induced different epitope-specific antibody response. Shrivastava T, Samal S, Tyagi AK, Goswami S, Kumar N, Ozorowski G, Ward AB, Chakrabarti BK. Vaccine. 2018 Mar 14;36(12):1627-1636. doi: 10.1016/j.vaccine.2018.01.081. Epub 2018 Feb 9.
• Characterization of a stable HIV-1 B/C recombinant, soluble, and trimeric envelope glycoprotein (Env) highly resistant to CD4-induced conformational changes. Kumar R, Ozorowski G, Kumar V, Holden LG, Shrivastava T, Patil S, Deshpande S, Ward AB, Bhattacharya J. J Biol Chem. 2017 Sep 22;292(38):15849-15858. doi: 10.1074/jbc.M117.803056. Epub 2017 Jul 25.
• Identification and characterization of a naturally occurring, efficiently cleaved, membrane-bound, clade A HIV-1 Env, suitable for immunogen design, with properties comparable to membrane-bound BG505. Das S, Boliar S, Samal S, Ahmed S, Shrivastava T, Shukla BN, Goswami S, Bansal M, Chakrabarti BK. Virology. 2017 Oct;510:22-28. doi: 10.1016/j.virol.2017.07.001.
• Stabilization of a soluble, native-like trimeric form of an efficiently cleaved Indian HIV-1 clade C envelope glycoprotein. Ahmed S, Shrivastava T, Kumar N, Ozorowski G, Ward AB, Chakrabarti BK. J Biol Chem. 2017 May 19;292(20):8236-8243. doi: 10.1074/jbc.M117.776419.
• Association of mutations in V3C3 domain with shift in evasion of HIV-1 clade C from follow up broadly neutralizing contemporaneous plasma antibodies in an elite neutralizer. Suprit Deshpande*, Shilpa Patil*, Rajesh Kuma*, Tripti Shrivastava et al. Retrovirology. 2016; 13: 41. doi:  10.1186/s12977-016-0273-x
• Conformational Epitope-Specific Broadly Neutralizing Plasma Antibodies Obtained from an HIV-1 Clade C Infected Elite Neutralizer Mediate Autologous Virus Escape through Mutations in V1 Loop. Shilpa Patil¶, Rajesh Kumar¶, Suprit Despande¶, Sweety Samal, Tripti Shrivastava, Saikat Boliar, et al.Journal of Virology. 2016 Jan 13. pii: JVI.03090-15
• An efficiently cleaved and native-conformation HIV-1 clade C Env that selectively binds to neutralizing antibodies. Saikat Boliar*, Supratik Das*, Manish Bansal, Brihaspati N Shukla, Shilpa Patil, Tripti Shrivastava, Sweety Samal, Sandeep Ghoswami, Richter C King, Jayanta Bhattacharya and Bimal K Chakrabarti. PLoS One, 2015 Mar 30; 10 (3):e0122443. doi: 10.1371/journal.pone.0122443
• Structural basis of Ets1 activation by Runx1. Tripti Shrivastava, Nigar Babayeva, Oxana Baranosky, Tahir H Tahirov. Nature - Leukemia, 2014 Oct; 28(10):2040-8.
• Three-Dimensional Structures of DNA-Bound Transcriptional Regulators, Tripti Shrivastava and Tahir H. Tahirov. Methods in Molecular Biology, 2010;674:43-55. Review
• Ligand-Induced Structural Transitions, Mutational Analysis, and ‘Open’ Quaternary Structure of the M. tuberculosis Feast/Famine Regulatory Protein (Rv3291c). Tripti Shrivastava, Abhishek Dey and Ramachandran, Ramachandran. Journal of MolecularBiology, 2009, 392, 1007–1019
• Mechanistic insights from the crystal structures of a feast/famine regulatory protein from Mycobacterium tuberculosis H37Rv. Tripti Shrivastava and Ravishankar Ramachandran. Nucleic Acid Research, 2007, 35 (21): 7324-35
• Cloning, expression, purification and crystallization of a transcriptional regulatory protein (Rv3291c) from Mycobacterium tuberculosis H37Rv, Tripti Shrivastava,S. K. Srivastava, and Ravishankar Ramachandran. Acta Crystallograph Sect D Biological crystallography, 2004, D 60, 1874 – 76

• Shuja S. Malik and Tripti Shrivastava. Protein characterization using modern biophysical techniques. Advances in Protein Chemistry, OMICS Group Incorporation

·         Patents

1. Indian Patent Application No.: 202021015307

Title: DNA CONSTRUCT EXPRESSING RECEPTOR BINDING DOMAIN OF SARS-COV-2 PROTEIN, THE PROCESS TO PRODUCE THE SAME AND ITS USES THERE OF

Dr Tripti Shrivastava, Sandeep Goswami

2.    Indian Patent Application No.: 20191 1027069

Title: A H1N1 Influenza virus M2e ectodomain protein obtained from an arrangement of M2e ectodomain polypeptides and uses thereof

Dr. Sweety Samal, Dr . Rajesh Kumar, Dr . Tripti Shrivastava, Naresh Kumar, Manish Bansal

3.    International Application No.: PCT/US2015/057098

Pub. No.: WO/2016/065252

Title:NATIVE TRIMERIC ENV IMMUNOGEN DESIGN

Bimal  Chakrabarti,  Saikat  Boliar,  Supratik  Das,  Tripti  Shrivastava,  Richte  Charles  King,  Jayanta  Bhattacharya, Sweety Samal.

• 2018    Travel Award for HIV R4P 2018, Madrid, Spain
• 2016    Travel Award for HIV R4P 2016, Chicago, USA
• 2008    “Dr D. L. Shrivastava” Young Scientist Award, Society of Biological Chemistry, India.
• 2007    Best Publication Award, Central Drug Research Institute, Lucknow, India.
• 2005    Senior Research Fellowship from Council of Scientific and Industrial Research, India.
• 2003    Graduate Aptitude Talent Examination (GATE, 98.52 Percentile)
• 2002    Junior Research Fellowship from Council of Scientific and Industrial Research, India.
• 2001    Cleared eligibility for lectureship from Council of Scientific and Industrial Research, India.

PDB DEPOSITION

• 2IVM- Crystal structure of a transcriptional regulator
• 2VC1, 2VC0, 2VBZ, 2VBY, 2VBX, 2VBW, 2W24 - Feast or famine regulatory protein (Rv3291c) from M. Tuberculosis complexed with amino acids
• 2W25, 2W29 - Crystal structure of mutant Protein (Glu104Ala and Gly102Thr)
• 4L0Y, 4L0Z, 4L18- Crystal structure of Runx1 and Ets1 bound to TCR alpha promoter (crystal form 1, 2 & 3) 

MEMBERSHIP OF PROFESSIONAL ORGANIZATION

• Life Member of Indian Crystallographic Association (ICA) (LM 609)