PROFILE PREVIEW

Research Interests

A. Diagnostics for Antimicrobial Drug Resistance (AMR)

Novel biomarker discovery, rapid pathogen capture & culture, pathogen identification and phenotypic antimicrobial drug resistance/suceptibility testing 

B. Mammalian Bioprocessing 

Host cell line development, recombinant protein production and process optimization

Research Experience

a. Postdoctoral Researcher at Institute of Applied Microbiology, BOKU, Vienna, Austria

Area of Research

- Cell line and Process development (collaborative project with Pfizer and Lonza Biologics)

- Biomarker discovery for regulated cell growth

b. Postdoctoral Researcher at Dublin City University, Dublin, Ireland

Area of Research

- Cell line and Process development (collaborative project with Pfizer)

- Biomarker discovery for breast cancer

• Singh R, Fatima E, Thakur L, Singh S, Ratan C, Kumar N. Advancements in CHO metabolomics: techniques, current state and evolving methodologies. Frontiers in Bioengineering and Biotechnology. 2024.
• Singh R, Maurya N, Tripathi K, Singh UP, Agrawal V, Goel A, Singhai A, Kumar N, Garg M. Pilot study on quantifying the epithelial/mesenchymal hybrid state in the non-muscle invasive and muscle invasive bladder tumors: A promising marker of diagnosis and prognosis. Advances in Cancer Biology-Metastasis. 2023.
• Singh R*, Thakur L*, Kumar A, Singh S, Kumar S, Kumar M, Kumar Y, Kumar N. Comparison of freeze-thaw and sonication cycles-based methods for extracting AMR-associated metabolites from Staphylococcus aureus. Frontiers in Microbiology. 2023. (*co-authors)
• Thakur L*, Singh S*, Singh R, Kumar A, Angrup A, Kumar N. The potential of 4D’s approach in curbing antimicrobial resistance among bacterial pathogens. Expert Review of Anti-infective Therapy. 2022. (*co-authors)
• Kumar A, Singh S, Gupta SK, Kumar S, Kumar S, Singh R, Thakur L, Kumar M, Kapil A, Kumar Y, Kumar N. Identification of metabolite extraction method for targeted exploration of antimicrobial resistance associated metabolites of Klebsiella pneumoniae. Scientific Reports. 2022
• Kaur J, Rawat Y, Sood V, Periwal N, Rathore DK, Kumar SK, Kumar N, Bhattacharyya S. Replication of Dengue virus in K562-megakaryocytes induces suppression in the accumulation of reactive oxygen species. Frontiers in Microbiology. 2022
• Gupta P, Kumar N, Garg M. Emerging roles of autophagy in the development and treatment of urothelial carcinoma of the bladder. Expert Opin Ther Targets. 2021
• Kumar A, Singh R, Kaur J, Pandey S, Sharma V, Thakur L, Sati S, Mani S, Asthana S, Sharma TK, Chaudhuri S, Bhattacharyya S, Kumar N. Wuhan to World: The COVID-19 Pandemic. Front. Cell. Infect. Microbiol. 2021
• Kumar A, Das B*, Kumar N*. Vibrio Pathogenicity Island-1: The Master Determinant of Cholera Pathogenesis. Front. Cell. Infect. Microbiol. 2020 (*co-corresponding)
• Kumar A, Kumar Y, Sevak JK, Kumar S, Kumar N and Gopinath SD. Metabolomic analysis of primary human skeletal muscle cells during myogenic progression. Scientific Reports, 2020 (*co-corresponding)
• Kumar N, Gupta DG, Kumar S, Maurya P, Tiwari A, Mathew B, Banerjee S, Haldar S, Pillai P, Bhatnagar S* and Chaudhuri S*. Exploring Packaged Microvesicle Proteome Composition of Chinese Hamster Ovary Secretome. J Bioprocess Biotech. 2016. (*Contributed equally).
• Chaudhuri S, Maurya P, Kaur M, Tiwari A, Borth N, Bhatnagar S*, Kumar N*. Investigation of CHO Secretome: Potential Way to Improve Recombinant Protein Production from Bioprocess. J Bioprocess Biotech. 2015. (*Contributed equally).
• Sharma C, Sankhyan A, Sharma T, Khan N, Chaudhuri S, Kumar N, Bhatnagar S, Khanna N, Tiwari A. A repertoire of high-affinity monoclonal antibodies specific to S. typhi: as potential candidate for improved typhoid diagnostic. Immunol Res. 2015.
• Kumar N, Borth N. Flow-cytometry and Cell sorting: An efficient approach to investigate productivity and cell physiology in mammalian cell factories. Methods. 2012.
• Taschwer M, Hackl M, Hernández Bort JA, Leitner C, Kumar N, Puc U, Grass J, Papst M, Kunert R, Altmann F, Borth N. Growth, productivity and protein glycosylation in a CHO EpoFc producer cell line adapted to glutamine-free growth. J Biotechnol. 2012.
• Bort JA, Hackl M, Höflmayer H, Jadhav V, Harreiter E, Kumar N, Ernst W, Grillari J and Borth N. Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures. Biotechnology Journal. 2011.
• Barron N*, Kumar N*, Sanchez N, Doolan P, Clarke C, Meleady P, O'Sullivan F, Clynes M. Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7. J Biotechnol. 2011. (*Contributed equally).
• Kumar N, Sanchez N, Gammell P, Dowling P, Henry M, Meleady P, Barron N, Clynes M. Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture. ESACT Proceedings (Springer Publication). 2009.
• Barron N, Kumar N, Sanchez N, Gammell P, Clynes M. MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes. ESACT Proceedings (Springer Publication). 2009.
• Kumar N, Gammell P, Meleady P, Henry M, Clynes M. Differential protein expression following low temperature culture of suspension CHO-K1 cells. BMC Biotechnol. 2008.
• Kumar N*, Maurya P*, Gammell P, Dowling P, Clynes M, Meleady P. Proteomic profiling of secreted proteins from CHO cells using Surface-Enhanced Laser desorption ionization time-of-flight mass spectrometry. Biotechnol Prog. 2008. (*Contributed equally).
• Kumar N, Gammell P, Clynes M. Proliferation control strategies to improve productivity and survival during CHO based production culture: A summary of recent methods employed and the effects of proliferation control in product secreting CHO cell lines. Cytotechnology. 2007.
• Gammell P, Barron N, Kumar N, Clynes M. Initial identification of low temperature and culture stage induction of miRNA expression in suspension CHO-K1 cells. J Biotechnol. 2007.

Book: 

Maurya P and Kumar N. Proteomics tools in Breast Cancer Research. Lambert Academic Publishing House. 2012.

• Kumar N, Sharma C, Chaudhuri S, Rathore DK, Kumar S, Pillai J, Bhatnagar S. Flow-cytometry based rapid method of testing antimicrobial drug susceptibility and resistance in biological fluids. Application No.: 201711046980. 

• Kumar N, Chaudhuri S, Tiwari A. Method of improving stability of recombinant protein product in CHO batch culture by selective removal of metalloproteinase rich microvesicular fractions and uses thereof. 2290/DEL/2015 (Granted)

• AMR Diagnostics: Development of test for rapid pathogen identification and phenotypic antimicrobial susceptibility testing using biological samples

• Mammalian Bioprocessing: Improving the recombinant protein production capability of host cell line (CHO cells) using reverse engineering based approaches

• Won second prize as Faculty-Entrepreneur under Faculty-lead Start-up Category at National Start-up Summit 2017 organized at India International Science Festival 2017, Chennai by the Government of India and Vijnana Bharati (VIBHA) along with other ministries/departments including AICTE, DST, DBT, CSIR, ISRO, DAE, DRDO and ICAR. 

Co-Founder Director

Tritek Innovation Pvt. Ltd. (a first faculty start-up under any DBT institution aimed to translate diagnostic research leads into product and/or services for human welfare)

Web: www.tritekinnovation.com

1. MS. RITA SINGH, PH.D STUDENT
2. MR. LOVNISH THAKUR, PH.D STUDENT
3. Mr. SEVARAM SINGH, PH.D STUDENT
4. MS. ERAM FATIMA, PH.D STUDENT
5. Mr. CHANDRA RATAN, PH.D STUDENT
6. MR. SHAILESH KUMAR, LAB TECHNICIAN
7. MR. MANOJ KUMAR, LAB TECHNICIAN